Shadow
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| SHW-101AP | Shadow N-terminal antibodies | Rabbit | AP | Drosophila Melanogaster | 100.0 ug | 150-500 ul |
$245.00
|
| SHW-112AP | Shadow C-terminal antibodies | Rabbit | AP | Drosophila Melanogaster | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-SHW100 | FITC-conjugated N-terminal antibodies | Rabbit | FITC-AP | Drosophila Melanogaster | 100.0 ug | 150-500 ul |
$350.00
|
| FITC-SHW110 | FITC-conjugated C-terminal antibodies | Rabbit | FITC-AP | Drosophila Melanogaster | 100.0 ug | 150-500 ul |
$350.00
|
| P-SHW100 | N-terminal antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| P-SHW110 | C-terminal antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-SHW | WB positive control for SHW | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
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F
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ruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog & wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promote its cell division in the female germline (1, 2). The Signaling by TGF beta-related factors requires ligand-induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. Steroid hormones play an important role in the reproduction, development and homeostasis between transitions between various stages of development (1). In D melonogaster the hormone ecdysone (E) is converted to 20-hydroxyacdysone by a P450 enzyme in peripheral target tissue. The genomic and regulatory effect of this hormone are mediated by activation of a nuclear hormone receptor an activation of transcriptional cascade (2). Recently it has been shown that a product of the Disemboided locus as being potentially involved in the production control of ecdysteroid as a member of the cytochrme P450 family monooxygenese (CYP302A). Mutation in the Disemboided protein result in a number of distinct phenotypes in D. melonogaster including disruption in dorsal closure, head evolution, gut morphogenesis, and failure to produce embryonic cuticle. Most of these disruptions are the result of low affinity of 20E to its receptor due to significant reduction in the expression of IMP-E1 gene. Several other mutations in the “Hawloween” group of proteins, including Shadow (sad) also resulted in similar phenotypes (3, 4). The co-expression of dib and sad causes increased metabolism of ketotriol to ecdyson (2) and the two proteins are concentrated with in the individual segment (2).
The Anti-Shadow-selective antibodies were generated against conserved sequences near the N-terminus and the C-terminal end of the protein that are unique to shadow protein. The Shadow-selective antibodies label the two variants of shadow proteins in fruitfly extracts. The Shadow antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The Saxophone antibodies label a 63-65kDa protein in D. melanogaster extracts. Anti-Shadow-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses. FabGennix Inc. will also conjugate antibodies with fluorescent probes upon request at extra charge.
FabGennix Inc. also provides several antibodies against proteins that are involved Drosophila melanogaster research such as several Anti-PDE antibodies, Anti-Wishfulthinking, Anti-Baboon, Anti-Shade, Anti-Saxophone, Anti-SARA anchor protein, Anti-Phantom and Anti-Punt protein. FabGennix Inc employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (6). FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available. Please enquire for their availability before ordering.
For research use only, not for diagnostic or therapeutic use.
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