Argonaute protein 1
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| Ergo1-101AP | Argonaute protein 1 antibodies | Rabbit | AP | C. elegans | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-Ergo1 | FITC-conjugated antibodies | Rabbit | FITC-AP | C. elegans | 100.0 ug | 150-500 ul |
$350.00
|
| P-Ergo1 | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-Ergo1 | WB positvie control for Ergo1 | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
In mammalian cells miRNA-induced silencing complexes (miRISC) are formed by microRNAs, which regulate protein expression post transcriptionally through binding to 3’-untranslated regions of the target mRNAs. At least 4 Argonaute proteins (Ergo1-4) have been identified. Recent studies have identified a conserved WG/GW-containing motif, known as the Argonaute (AGO) hook, which is involved in the recruitment of AGOs to distinct components of the eukaryotic RNA silencing pathways. Argonaute proteins are important component of the miRISC complexes. Argonaute 2 (Ergo-2) recruits GW182, a key component of miRISC of the processing body (GW/P-body) to target mRNA. Ergo-2 interacts with GW182 at three binding sites within the amino terminal GW/GW)-repeat region that is characteristics of the GW182 family proteins. It is been shown that multiple Ergo proteins are connected via GW182 proteins. The interaction of Ergo with GW182 family proteins relieved let-7-mediated repression of protein synthesis in mammalian cell-free system, as a result of delayed let 7-directed target mRNA deadenylation (1). These data suggest that that the interactions of GW182 with Argonautes may induce the formation of large complexes containing miRNA target mRNAs, and may be critical for miRNA-mediated translational repression.
The 97-megabase genomic sequence of the nematode Caenorhabditis elegans reveals over 19,000 genes. More than 40 percent of the predicted protein products find significant matches in other organisms. There is a variety of repeated sequences, both local and dispersed. The distinctive distribution of some repeats and highly conserved genes provides evidence for a regional organization of the chromosomes (2). One of the protein sequenced from Caenorhabditis elegans was Endogenous-RNAi-deficient Argonaute family member protein 1 (ergo-1). The protein comprise of 112 amino acids, calculated MW of 126.5kDa, with several function conservative domains. This protein is part of the RNA-induced silencing complex (RISC) and is an endonuclease that plays a key role in RNA interference pathways. The PAZ domain (amino acid 462-533) was named after protein containing Piwi, Argonaute and cd02846. The Piwi domain is the c-terminal portion of the Argonaute protein and contains two sub domains. The protein also has a nucleic acid binding interface and a 5’RNA guide strand anchoring site. guide
The Anti-Endogenous-RNAi-deficient Argonaute family (Ergo-1) protein antibodies were generated against a synthetic peptide corresponding to C-terminal location of Ergo 1 protein from Caenorhabditis elegans. The Ergo 1-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded high affinity epitope-specific antibodies. The Ergo1 antibodies label a protein of approximately 127kDa Da in Ergo 1 western blot positive control samples (PC-ergo1). Anti-Ergo1-selective antibodies are also available in FITC-conjugated forms (FITC-Ergo1) for confocal, Western blotting and immunocytochemical analyses. FabGennix Inc. will also conjugate this and all its other antibodies with fluorescent probes upon request at a nominal extra charge. Limited quantities of antigenic blocking peptide and western blot positive control for Ergo1 are also available (please inquire their availability before ordering). FabGennix Inc. has produced and characterized antibodies for epigenetics and, cell cycle-related research areas, for a complete listing of these products, please visit www.FabGennix.com. FabGennix Int. Inc., provides custom antibody development services specially for post-translationally modified epitopes including phosphorylation, lipid modifications, glycosylations, oxidations etc.). Please inquire for their availability before ordering.
For research use only, not for diagnostic or therapeutic use.
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