P2Y2 nucleotide receptor 2
| Catalog# | Product Description | Host Species | Nature | Cross reactivity | Quantity | Volume | Price |
|---|---|---|---|---|---|---|---|
| P2Y2-201AP | P2Y2R antibodies | Rabbit | AP | r, m, h, cat | 100.0 ug | 150-500 ul |
$245.00
|
| FITC-P2Y2 | FITC-conjugated antibodies | Rabbit | FITC-AP | r, m, h, cat | 100.0 ug | 150-500 ul |
$350.00
|
| P-P2Y2 | Antigenic blocking peptide | n/a | Synthetic peptide | n/a | 250.0 ug | 100 ul |
$125.00
|
| PC-P2Y2 | WB positive control for P2Y2 | n/a | Protein in ready-to-use buffer | n/a | 0.0 ug | 150 ul |
$185.00
|
Purinenucletide receptor are classified in to two classes, the ionotrophic ligand-gated channels, P2X and the metabotropic G-protein coupled receptors, P2Y (1). There are 7 P2X receptors and 11 P2Y (P2Y1-2, 4, 6 and 11-14) each with unique agonist response profile has been identified. The subtypes of metabotropic receptors P2Y1 and P2Y2 are predominantly expressed in arterinal smooth muscle. The activation nof P2Y2 receptors led to release of intracellular calcium by phospholipase C action after which receptor phosphorylation appears to regulate desensitization of the P2Y2 receptor via action of protein kinase C. The purinergic receptors P2Y2 couples to the activation of mitogen activated protein kinases (MAPK) and mobilize the intracellular calcium stores, activation of phospholipase Cb, protein ikinase C isofroms, focal adhesion kinases and c-Src kinases. These pathways are implicated in number of physiological and pathological processes including inflammation, neuroprotection, active astrogliosis etc (1, 2). P2Y2 receptor induce chloride secretion in airway epithelial cells independent of cystic fibrosis transmembrane conductance regulator and this receptor ligands are therapeutically important for the treatment of retinal detachment and improved mucociliary clearance in cystic fibrosis patients.
The P2Y2 nucleotide receptor is seven TMD GPCR family member and is coupled to a Gq protein that is stimulated equipotently by UTP and ATP, mediating activation of phospholipase C-beta (PLC-β) and mitogen-activated protein kinase (MAPK). The P2y2 undergoes rapid agonist induce desensitization and phosphorylation at Serine 243, threonine 344 and serine 356 (1). The antagonist potencies for P2Y2 receptor is in the order of suramin>>PPADS= RB-2>TNP-ATP and suramin (pA2, 5.40) was a competitive antagonist (3). The extracellular acidification does not affect the ATP and UTP potencies of P2Y2 receptors while the P2Y4 receptor potencies are increased by 8-10-fold (3). Both P2Y2 and P2Y4 receptors regulate the UTP induced chloride secretion from intestinal mucosa and cuased endothelium induced relaxation in aorta (4, 5).
P2Y2 receptor when expressed in host cells showed significant heterogeneity on SDS-PAGE mobility like many other PGCRs (6). The P2Y2 is a 377 aa protein with characteristic 7TMDs of GPCR, exhibit a broad band od 52-76 kDa (7). The P2Y receptor sequence analyses suggest the presence of putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins and has several phosphorylation sites. The Anti-P2Y2-selective antibodies were generated against conserved but unique sequences on from P2Y2 gene that are expressed only in P2Y2 and was common in several other species. The polyclonal antibodies were affinity purified on an immobilized antigen based affinity chromatography. These epitope-spcific antibodies strongly labeled P2Y2 protein form P2Y2-western blot positive controls. Anti-P2Y2-selective antibodies are also available as fluorescent conjugates for direct application in IHC. FabGennix, Inc., will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigenic peptides are also available. Please enquire for their availability before ordering. FabGennix Int. Inc. also provides antibodies against several other GPCRs including many orphan receptors, for a complete listing visit www.fabgennix.com.
For research use only, not for diagnostic or therapeutic use.
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