The solute carrier family SLC35 consists of at least 17 molecular species in humans. The family members so far characterized encode nucleotide sugar transporters localizing at the Golgi apparatus and/or the endoplasmic reticulum (ER). These transporters transport nucleotide sugars pooled in the cytosol into the lumen of these organelles, where most glycoconjugate synthesis occurs (1). Various genetic defects have been attributed to various mutations and alteration in nucleotide and sugar transporters in small multi-cellular organisms. The defects in these transporters are also attributed to tumor metastasis , cellular immunity organogenesis and morphogenesis. The Leukocyte adhesion deficiency type II or the congenital disorder of glycosylation type II c are the sole human congenital disorder known to date are caused by defects in the Solute carrier family member 35 (GDP-fucose transporter). Genetic aberrations in Slc35E subtype family members are also attributed to connective tissue and muscle disorders.
Solute carrier transporter are membrane associated proteins with up to 10 trans-membrane domains (TMD). With the help of National Institutes of Health Mammalian Gene Collection (MGC) Program which is a multi-institutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and mouse gene. ESTs were generated from libraries enriched for full-length cDNAs and analyzed to identify candidate full-ORF clones, which then were sequenced to high accuracy. The MGC has currently sequenced and verified the full ORF for a non-redundant set of >9,000 human and >6,000 mouse genes. Candidate full-ORF clones for an additional 7,800 human and 3,500 mouse genes also have been identified. One of these many sequences represents a Slc35E family member (Slc35E4) with 10 TMD, a characteristic of solute carrier proteins. Slc35E4 family member has significant homology to phosphate-phosphoenolpyruvate translocator. This sequence was cloned form a biopsy from mammary tumor (metallothionien-TGF alfa model) of a 10 month old virgin mouse. The full length ORF for Slc35E4 is a 350 amino acid protein (apparent MW 43-45kDa), with characteristic 8 primary TMDs, the transcripts are expressed in multiple tissues and has high homology with Trios phosphate transporter (TPT-family) member.
The Scl35E4-selective antibodies were generated against a peptide corresponding to amino acid 172-195 residing on extracytosolic phase between TMD5 andTMD6. The peptide was covalently modified after synthesis to achieve desired antigenicty before coupling to a carrier protein. The Slc35E4-selective antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulin are stabilized in antibody stabilization buffer for long-term storage. Limited quantities of the antigenic blocking peptide for Slc35E4 antibodies are also available (please inquire for availability). FabGennix Inc. will conjugate antibodies with enzymes or fluorescent probes (FITC, Cy3, cy5, Rhodamine etc.,) as custom service upon request at a nominal cost. For easy identification of Slc35E4 on western blots, FabGennix also provide Western blot positive controls for Slc35E4 in ready-to-use buffer. FabGennix International Inc., has produced a number of antibodies for diagnostic and research use, please visit www.FabGennix.com for a complete listing.
For research use only, not for diagnostic or therapeutic use.
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