Arsenate activated ATPase

Accession #/Synonyms: NP_004308; ATPase ASNA1; Golgi to ER traffic protein 3, GET3, Arsenite stimulated ATPase, Guided entry of tail anchored protein 3.

Tail-anchored (TA) proteins are post-translationally targeted to and inserted into the endoplasmic reticulum (ER) membrane through their single C-terminal trans-membrane domain.  The TA protein insertion is mediated by ATPaseTRC40/Asna1(Golgi ER traffic protein 3, GET3 in yeast) and a receptor in ER membrane.  The Asna1/TRC40 binds to Tryptophan-rich binding protein (WRB) also known as congenital heart disease protein 5 (CHD5) as the ER membrane receptor for Asna1/TRC40 (2).  .  The bacterial Arsenical driving pump ATPase (Asna1) is the catalytic component of an oxyanion pump that is responsible for resistance to arsenicals and antimonials compounds. ATPases are required for the post-translational delivery of tail-anchored proteins to the endoplasmic reticulum for post synthetic modifications.  The arsenite activated ATPases recognizes and selectively binds the trans-membrane domain of TA protein sin the cytosol. This complex then targets to the endoplasmic reticulum by membrane bounds receptors where the t ail anchored protein is releases for insertion. The protein targeting in the ER is driven by ATP binding and hydrolysis. Asna1/TA protein complex can insert RAMP4 and Sec61 beta without the participation of of other cellular protein, whereas cytochrome b5 can be membrane bound without Asna 1 (3). .

Homologues of the bacterial ArsA ATPase are widespread in nature, a mouse homologue Asna1 exhibit 27% identity to the bacterial ArsA ATPase. Asna1 is an efflux pump to extrude Arsenite, antimonite and arsenate by an ATP driven process located on the cell membranes. The Ansa1 has several structural motifs including P-loop-NTPase that has nucleoside triphosphate hydrolase activity.  Fluorescence in situ hybridization suggests that the Asna1 gene is localized in the C3-D1 region of mouse chromosome 8 (3). The human homologue of Asna1 is a 348 aa long polypeptide with approximate Mw of 42kDa.  .

Asna1- selective antibodies were generated against a peptide taken form near C-terminal region spanning human Asna1 300-348aa. The 20mer peptide was conserved in rat, mouse and human Asna1.  The antibodies do not cross react to other ATPAses. The anti-Asna1-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions.  The Asna1antibodies are affinity purified on an immobilized antigen based affinity chromatography; the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage.  FabGennix Int. Inc. provides Asna1 Western blot positive control samples in “ready-to-use” SDS-PAGE sample buffer.  Limited quantities of antigenic blocking peptide for Asna1antibody is also available, please inquire before ordering.  FabGennix Int. Inc., will also conjugate this antibody to fluorophores and other secondary enzymes at a nominal price. FabGennix Int. Inc., provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected. For a complete listing of all FabGennix antibodies please visit www.Fabgennix.com. 

For research use only, not for diagnostic or therapeutic use.