Far upstream-element binding protein 1

Accession #/Synonyms: AAH17247; Fubp1; Far upstream binding (FUSE) protein 1 (FUBP1);   .

Nucleophosmin (NPM/B23) is a multifunctional oncoprotein whose protein expression levels dictate cellular growth and proliferation rates.  The 3’ untranslated region (UTR) of the NPM messenger RNA is sufficient to modulate its translation by mTOR signaling mainly due to binding of far upstgream element (FUSE) binding protein (FUBP1). Over expression of FUBP1resulted in to translational repression of NPM mRNA , whereas depletion of FUBP1 caused a dramatic increase in NPM expression and resulted in to enhanced cell proliferation (1).  FUBP1 is a key regulator of the cell proliferation and growth via its ability to selectively bind the NPM 3’ UTR region. Human c-myc expression is also under regulation  by an activator FUBP1 and a repressor, the FUBP1-intercting repressor FIR (2). Under physiological condition, FIR is always in monomeric form but upon binding with FUBP1 it forms a complex FIR2-FUBP1 mediated by RRMs (3). 

The FUBP1 and CIC (drosophila gene capicua) genes have shown mutation resulted in formation of truncated from of CIC and FUBP1 in oligodendroglioma tumors.  These results suggest FUBP1 and CIC genes may play critical role in pathology of oligodenroglioma (3).  The FUBP1 gene is located on chromosome 19q .  At least three Far upstream-element binding proteins (FUBP1-3) have been characterized to bind specifically to TG microsatellite in the untranslated 3’UTR region.  The protein encoded by FUBP1 binds to single stranded DNA, in particular the far-upstream element (FUSE) of MYC, a well-studied oncogene. 

MUBP1- selective antibodies were generated against peptides form unique region on FUBP1 protein corresponding to amino acid 626-642 near the C-terminal end of protein. This region was not only antigenic but also lies on the surface of the protein in native or in partially denatured FUBP1 confirmation.  The anti-FUBP1-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions.  The FUBP1antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage.  FabGennix Int. Inc. provides FUBP1Western blot positive control samples in “ready-to-use” SDS-PAGE sample buffer.  Limited quantities of antigenic blocking peptide for FUBP1antibody is also available, please inquire before ordering.  FabGennix Int. Inc., will also conjugate this antibody to fluorophores and other secondary enzymes at a nominal price. FabGennix also provides custom antibody production services for researchers that are looking for high affinity mono and polyclonal antibodies in various species. We specialize in making application specific antibodies that are useful in IHC, confocal and other applications where native antigen is detected. For a complete listing of all FabGennix antibodies please visit www.Fabgennix.com. 

For research use only, not for diagnostic or therapeutic use.