Netrin-1 (Ntrn1) also known as axonal guidance protein (Ntrn1) is a diffuse protein made by the floor plate cells that belongs to the netrin family of proteins. Netrin family of proteins guides axons to or away from the midline of the developing nervous system (1). In mammals, netrins act as ligands for DCC (deleted in colorectal cancer) receptor and the UNC5 receptor homologues: UNC5A, B, C and D. DCC mediates chemoattraction whereas repulsion is mediated by UNC5 homologues and, in some cases, DCC (2). Netrin-1, located at human chromosome 17p13-p12, attracts spinal commissural axons and repels trochlear axons in vitro. In mice, deficiencies in Netrin-1 led to defects in spinal commissural axon projections (3). Netrin-1 also acts as an early ventral spinal cord-derived chemorepellent for dorsal root ganglion (DRG) axons. In the dorsal and intermediate spinal cord, it prevents DRG axons from being directed towards the dorsal spinal cord (4). Netrin-1 and its associated receptors play a key role cell death and tumor formation.
Netrin-1/receptor pair induces apoptosis in cultured cells and also acts as regulators of tumor development (5, 6). These properties of Netrin-1 have been implicated in colorectal tumorigenesis by regulating apoptosis (6). In the embryo, Netrin-1 is widely expressed in the developing nervous system and in mesodermal tissues. In adults, it is highly expressed in heart, small intestine, colon, liver and prostrate. It is also expressed in brain tumors and neuroblastomas. Netrin-1 is approximately a 73kDa protein (604 amino acids).
The Netrn1-selective antibodies were generated against synthetic peptide taken from near C-terminal end of the Netrin-1 (Netrn1) protein. Netrn1-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc., will also provide limited quantities of antigenic blocking peptide/protein for Ntrn-101AP antibody. Antibodies to Netrn1 (Ntrn-101AP) will label ~73kDa protein in Western blot positive control samples for Netrn1 and several other tissues. FabGennix Inc. will also conjugate antibodies with secondary enzymes (alk-Pase or HRP) or fluorescent probes upon request at nominal cost. FabGennix has made many new antibodies related to Neurosciences, for a complete listing visit www. FabGennix.Com.
For research use only, not for diagnostic or therapeutic use.
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Netrin-1
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