Collapsin response mediated protein 2

Alternate Nomenclature:NP_001378Dihydropyrimindase-related protein 3 isoform 2 ; TOAD65, Turn on after division 65 protein.

Collapsing mediated response proteins (CRMPs) form homo- and hetero tetramers and facilitate neuron guidance, growth and polarity. It is abundantly expressed in brain and regulate neurite outgrowth.  The CRMP family consists of 5 subtypes (CRMP1-5) and are also known as dihydroxypyrimindase 1-5 (DRP1-5).  The CRMPs promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse and also plays a role in synaptic signaling through interactions with calcium channels.  CRMP2 protein regulates endocytosis where two proteins MICAL-L1 and EHD1 are important regulatory proteins that control key endocytotic transport steps. CRMP2 interacts with MICAL-L1 in non-neuronal cells (1).  CRMP2 interacts with tubulin dimers and kinesin and negatively regulates s dynein-based transport in neuronal cells (2). 

The mammalian target of rapamycin (mTOR)is an important regulator of neuronal development and function. mTOR inhibitor, rapamycin regulates neuronal polarity by suppressing the expression of Tau and CRMP2 via mTOR effector 70-kDa ribosomal protein S6 kinase (p70S6K)p70 (2). It is suggested that abnormal activation of CDK5 is associated with neurodegeneratative disorders and it disrupts mature neuronal circuitries and neurogenesis. CDK5 and other kinases hyper-phosphorylate down-stream targets of these kinases in brain such as doublecortin, nestin, and CRMP2 (2). This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer’s disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

CRMP2-selective antibodies were generated against peptides form unique region near N-terminus of CRMP2 protein with in aa 1-100.  The epitope is conserved in several other species beside human but was unique to CRMP2.  The CRMP2 antibodies are affinity purified over immobilized antigen based affinity chromatography and fully characterized for their listed applications and cross reactivity.  The CRMP2 antibodies are also available as FITC-conjugates, other types conjugates can be ordered at a nominal charge. FabGennix Int. Inc., also provides western blot positive controls and antigenic blocking peptide for this antibody.  Please inquire for pricing and availability.  FabGennix International Inc., has produced antibodies to a wide range of targets, for a complete listing please visit www.FabGennix.com.  FabGennix antibodies, reagents and kits are now available for sale via our worldwide distribution centers. 

For research use only, not for diagnostic or therapeutic use.